Team:UNITN-Trento/Notebook/Labposts/06/07

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Latest revision as of 09:35, 3 October 2013

{ "date" : "2013-06-07", "author" : "viola-fabio-emil", "title" : "Purification and PCR of pSB1C3 linearized, gel", "content" : "We purified with the Quick Reference purification kit(GE) and made a PCR of the linearized plasmid pSB1C3 using the prefix and suffix primers and the Phusion polimerase following its protocol. Then we took the PCR samples and checked the results with an electrophoresis gel using the ladder Generuler 1 kb Plus DNA Ladder of Fermentas:

Gel Image

As we can see the plasmid, stopped at 2 kb ca (pSB1C3=2070kb).", "tags" : "pSB1C3" }