Team:UNITN-Trento/Notebook/Labposts/07/41

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(Difference between revisions)

Latest revision as of 10:41, 3 October 2013

{"date" : "2013-07-19","author" : "caterina","title" : "SAM sythetase and other miniprep","content" : "Yesterday, I performed inocula from different plates prepared by Gire. After miniprep and screening we had the confirmation that into pSB1C3 there was RFP and not SAM synthetase. So on Monday we will repeat the experiment.

Then I performed the miniprep of some inocula that I prepared the yesterday containing K1065101 and K1065102.","tags" : "SAM-K1065101-K1065102"}

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+{"date" : "2013-07-19","author" : "caterina","title" : "SAM sythetase and other miniprep","content" : "<html>Yesterday, I performed inocula from different plates prepared by Gire. After miniprep and screening we had the confirmation that into pSB1C3 there was RFP and not SAM synthetase. So on Monday we will repeat the experiment.<br/><br/>Then I performed the miniprep of some inocula that I prepared the yesterday containing K1065101 and K1065102.</html>","tags" : "SAM-K1065101-K1065102"}
-	"date" : "2013-07-21",
-	"author" : "thomas",
-	"title" : "Ligation of AraC-pBAD + EFE and Venus",
-	"content" : "<html>This is the part three of <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-18-thomas-michele\">this experiment</a>. The digestion products were ligated together following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Ligation\">this protocol</a>. The vector (AraC-pBAD + EFE) was ligated with 1:0 (control), 1:1 and 1:4 of insert (Venus). The ligation product was then transformed into NEB10b competent cells and plated on Cloramphenicol containing Petri dishes.</html>",
-	"tags" : "EFE-Venus"
-}