Team:UNITN-Trento/Notebook/Labposts/07/56

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(Difference between revisions)

Latest revision as of 10:45, 3 October 2013

{"date" : "2013-07-24","author" : "fabio-bruno","title" : " the blue ligation: the never ending story !","content" : "Today I inoculated 6 colonies of the plated ligation ( 3 from the 1:1 plate and 2 from the 1:3 plate)
I also made the Onetaq Pcr for all the light parts ( blue device,the b sample from the first set; red device, from the third set; and red promoter, from the third set).
After that I screened them all and found out that only the blue part was confirmed: up to now we only have the blue parts confirmed!! Red parts , it seems to me that you’re in hot waters !!

Immagine gel

","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"}

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+{"date" : "2013-07-24","author" : "fabio-bruno","title" : " the blue ligation: the never ending story !","content" : "<html>Today I inoculated 6 colonies of the plated ligation ( 3 from the 1:1 plate  and 2 from the 1:3 plate) <br>I also made the Onetaq Pcr for all the light parts ( blue device,the b sample from the first set; red device, from the third set; and red promoter, from the third set). <br>After that I screened them all and found out that only the blue part was confirmed: up to now we only have the  blue parts confirmed!! Red parts , it seems to me that you’re in hot waters !! <br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Immagine gel|<html><center><img src=\"https://static.igem.org/mediawiki/2013/7/7f/Tn-2013_Daa.jpg\" width=\"450px\" /></center></html>}}<html></html>","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"}
-	"date" : "2013-07-27",
-	"author" : "fabio",
-	"title" : " the blue ligation: the never ending story !",
-	"content" : "<html> I first added sap to the o/n digestion( third), then purified and quantified : yield, 32,3 ng/ul.Then again I ligated 006 to 016 folowing the protocol and plated 10 ul in 200 ul of neb10b.In the afternoon I made 6 inocula from the second ligation plates!Today I decided also to take a crack at a fourth ligation with a new strategy: using 006 as my plasmid and 016 as the insert!! I need to digest the two part with different enzymes E and X for 006 and E and S for 016! This time I digested 2400 ng for only 5 ours, not all the night: yields are, 25 ng/ul for 016 and 11,8ng/ul for oo6. Tomorrow I will continue with the ligation number 4.</html>",
-	"tags" : " YF1_FixJ - FixK2"
-}