From 2013.igem.org
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| - | { | + | {"date" : "2013-07-26","author" : "gabriele","title" : "Re-screening","content" : "<html>I performed again the screening on Caterina's samples from 17/07 but instead of AgeI, which is wrong, I used BamHI-HF and PstI-HF. The screening was negative... :(</html>","tags" : "SAMsynthetase"} |
| - | "date" : "2013-07-30",
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| - | "author" : "thomas",
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| - | "title" : "Bacillus transformation!!!",
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| - | "content" : "<html>In order to test the transformation protocol for <i>B. subtilis</i> that Groeningen team kindly sent to us I prepared a competent cell Media following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#subtilis-transformation\">this protocol</a>. I proceeded then perfonming the transformation using the empty vector BBa_K823026. I finally plated the tranformed cells into LB agar plates with 10 ug/ml of Kanamycin. The day after took a picture of the plate and performed a Gram staining following <a href=\"\">this protocol</a>. </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plate image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/e/ec/Tn-2013_bacillus_transformation_plate_1.jpg\" style =\"width: 450px\"></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Bacillus microscope image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/6/65/Tn-2013_gram_staining_bacillus.jpg\" style =\"width: 450px\"></center></html>}}<html> As you can see from the pictures, seems that <i>Bacillus</i> was successful transformed!!!!</html>",
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| - | "tags" : "b. subtilis-pspac"
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| - | } | + | |
Latest revision as of 10:46, 3 October 2013
{"date" : "2013-07-26","author" : "gabriele","title" : "Re-screening","content" : "I performed again the screening on Caterina's samples from 17/07 but instead of AgeI, which is wrong, I used BamHI-HF and PstI-HF. The screening was negative... :(","tags" : "SAMsynthetase"}
"
