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Team:Heidelberg/Templates/MM week16p
From 2013.igem.org
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Nils.kurzawa (Talk | contribs)
(Created page with " == 2013-08-12 == * sequences arrived: File:PIK1.2-pIK2.2 seq 2013-08-12.zip * perform multiple sequence alignment against assumed sequences: [[:File:PIK1.2-2013-08-12 VF2.cl...")
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(Created page with " == 2013-08-12 == * sequences arrived: File:PIK1.2-pIK2.2 seq 2013-08-12.zip * perform multiple sequence alignment against assumed sequences: [[:File:PIK1.2-2013-08-12 VF2.cl...")
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Revision as of 17:09, 3 October 2013
2013-08-12
- sequences arrived: File:PIK1.2-pIK2.2 seq 2013-08-12.zip
- perform multiple sequence alignment against assumed sequences: pIK1.2 with VF2, pIK1.2 with VR, pIK2.2 with VF2, pIK2.2 with VR
- pIK1.2 looks good
- part of RBS missing in pIK2.2 -> makes no sense, as part of binding part of IK35, with which the backbone was amplified
- send pIK2.6 to sequencing with primers VF2 and VR, also sequence pIK1.2 with primer IK25 to find out whether RBS is also missing there
2013-08-13
- sequences arrived: File:PIK1.2-pIK2.6 seq 2013-08-13.zip
- perform multiple sequence alignment against assumed sequences: pIK1.2 with IK25, pIK2.6 with VF2, pIK2.6 with VR
- sequences look good
- prepare glycerol stock of TOP10-pIK1.2
2013-08-14
- prepare glycerol stock of TOP10-pIK2.6
