Team:TU Darmstadt/materials/Antarctic Phosphatase

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!align="center"|[[Team:TU_Darmstadt/labbook|<span style="color:white;font-size:160%;"> Labbook |</span>]]
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== Antarctic Phosphatase ==
== Antarctic Phosphatase ==
=== About ===
=== About ===

Latest revision as of 17:32, 3 October 2013

Labbook | Protocols | Materials |

Contents

Antarctic Phosphatase

About

Antarctic phosphatase catalyzes the removal of 5´ phosphate groups from DNA and RNA. Since phosphatase-treated fragments lack the 5´ phosphoryl termini required by ligases, they cannot self-ligate. This property can be used to decrease the vector background in cloning strategies.[1]

Working conditions

Buffer: 1X Antarctic Phosphatase buffer

Supplier

  • NEB http://www.neb.com/nebecomm/products/productM0289.asp

References

[1] http://www.neb.com/nebecomm/products/productM0289.asp

Retrieved from "http://2013.igem.org/Team:TU_Darmstadt/materials/Antarctic_Phosphatase"