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Team:DTU-Denmark/Notebook/10 July 2013
From 2013.igem.org
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* 3 samples from purification of 09.07.2013 | * 3 samples from purification of 09.07.2013 | ||
| - | ====Transformation | + | ====Transformation of Biobricks==== |
According to the iGEM protocol ([http://parts.igem.org/Help:Protocols/Transformation transformation protocol]) with slight changes. | According to the iGEM protocol ([http://parts.igem.org/Help:Protocols/Transformation transformation protocol]) with slight changes. | ||
* step 1: 100 μl cells | * step 1: 100 μl cells | ||
Revision as of 16:15, 10 July 2013
Contents |
208
Main purpose
Run gel with 9 PCR samples of Nir operon from Pseudomonas aeruginosa
Transformation in E.coli
Who was in the lab
Ariadni,Henrike,Julia,Natalia
Procedure
Run gel
- 6 samples from PCR of 09.07.2013
- ladder broadband
- 3 samples from purification of 09.07.2013
Transformation of Biobricks
According to the iGEM protocol ([http://parts.igem.org/Help:Protocols/Transformation transformation protocol]) with slight changes.
- step 1: 100 μl cells
- step 2: 1.5 μl of the resuspended DNA
- step 6: 90 sec (instead of 60 s)at 42 oC
- step 9: Incubation without shaking
