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Team:Tuebingen/Notebook/Protocols/tae
From 2013.igem.org
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<td style="text-align: center">57.1 mL</td> | <td style="text-align: center">57.1 mL</td> | ||
| - | <td>Glacial acetic | + | <td>Glacial acetic acid</td> |
</tr> | </tr> | ||
Revision as of 22:05, 3 October 2013
TAE Buffer
Back to Protocols
Reagents
| 242.0 g | Tris Base |
| 57.1 mL | Glacial acetic acid |
| 100 mL | EDTA (c = 0.5 M ; pH 8.0) |
| 10 mL | KCl (c = 250 mM) |
Procedure
Dissolve all reagents in 1000 mL Aqua bidest. Result: 50x concentration.
