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Team:Tuebingen/Notebook/Protocols/inoue
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Revision as of 23:36, 3 October 2013
Chemo-competent E. coli cells
Back to Protocols
Reagents
| 9.67 g | MnCl2 * 2 H2O |
| 2.2 g | CaCl2 * 2 H2O |
| 18.65 g | KCl |
| 20.0 mL | PIPES (c = 0.5 M ; pH 6.7) |
| to 1 L | Aqua dest. |
Procedure
Mix all reagents and fill up to 1 L with Aqua dest. Serilize through filtration (0.45 µm filter) and store at -20°C.
