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Team:Tuebingen/Notebook/Protocols/mini-prep
From 2013.igem.org
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<li>Resuspend cells in 250 µL Genaxxon Buffer 1.5 (with RNase)</li> | <li>Resuspend cells in 250 µL Genaxxon Buffer 1.5 (with RNase)</li> | ||
<li>Transfer cells to Eppendorf-tube.</li> | <li>Transfer cells to Eppendorf-tube.</li> | ||
| - | <li>Continue according to <a href="http://www.genaxxon.de/Katalog/DNA-Reinigungskits/Plasmid-DNA-Purification-Mini-Prep-Kit-50-columns.html">Genaxxon DNA Purification Mini Prep Kit Manual</a></li> | + | <li>Continue according to <a href="http://www.genaxxon.de/Katalog/DNA-Reinigungskits/Plasmid-DNA-Purification-Mini-Prep-Kit-50-columns.html">Genaxxon Plasmid DNA Purification Mini Prep Kit Manual</a></li> |
| - | <li>In the end, elute | + | <li>In the end, elute with30 µL Genaxxon Buffer 5.</li> |
</ol> | </ol> | ||
</div> | </div> | ||
Revision as of 02:12, 4 October 2013
Mini-Prep
Back to Protocols
Procedure
- Inoculate 10 mL LB-medium with one single colony from a fresh plate (e.g. after transformation) and incubate at 37 °C and 220 rpm over night.
- On the next day, transfer culture to 15 mL Falcon tube and centrifuge for 10 min at 3500 rpm
- Discard supernatant completely.
- Resuspend cells in 250 µL Genaxxon Buffer 1.5 (with RNase)
- Transfer cells to Eppendorf-tube.
- Continue according to Genaxxon Plasmid DNA Purification Mini Prep Kit Manual
- In the end, elute with30 µL Genaxxon Buffer 5.
