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Team:Tuebingen/Notebook/Protocols/gelextraction
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| - | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: | + | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> |
| + | <p> </p> | ||
| + | |||
| + | <h3>Procedure</h3> | ||
| + | |||
| + | <ol> | ||
| + | <li>Follow <a href="http://www.genaxxon.com/catalogue/DNA-Purification-Kits/PCR-and-Gel-extraction-Mini-Prep-Kit-50-columns.html">Genaxxon PCR and Gel extraction Mini Prep Kit Manual</a></li> | ||
| + | <li>Elute in 20 µL Genaxxon Buffer 3.2.</li> | ||
| + | <li>Run eluate twice through the column in order to increase DNA yields.</li> | ||
| + | <li>Control gelextraction via <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/gelelectrophoresis">gelelectrophoresis</a>.</li> | ||
| + | </ol> | ||
</div> | </div> | ||
Revision as of 02:25, 4 October 2013
Gelextraction
Back to Protocols
Procedure
- Follow Genaxxon PCR and Gel extraction Mini Prep Kit Manual
- Elute in 20 µL Genaxxon Buffer 3.2.
- Run eluate twice through the column in order to increase DNA yields.
- Control gelextraction via gelelectrophoresis.
