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Team:Tuebingen/Notebook/Protocols/lb
From 2013.igem.org
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<h3>Procedure</h3> | <h3>Procedure</h3> | ||
| - | <p> | + | <p>Inan autoclavable flask, dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. Let cool down and add antibiotics if required. Store at 4 °C.</p> |
</div> | </div> | ||
Revision as of 03:09, 4 October 2013
LB Medium
Back to Protocols
Reagents
| 10.0 g | Trypton |
| 5.0 g | Yeast extract |
| 10.0 g | NaCl |
| Antibiotics |
Procedure
Inan autoclavable flask, dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. Let cool down and add antibiotics if required. Store at 4 °C.
