Team:EPF Lausanne/Next steps

From 2013.igem.org

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Up until now we didn't succeed to make the proof of principle, but with a bit more time, we would have done the following experiments to characterize our parts and assemble them.
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Up until now we succeded
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• Producing Nanoparticles and loading them
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• Clone a pH sensitive promoter
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• Engineer a fusion protein between ice nucleation protein and Streptavidin
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But we weren’t able to characterize and assemble the parts completely.
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==What we would have done with more time:==
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==Nanoparticles==
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•
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•
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•
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==Cell surface display of Streptavidin==
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• Cloned a plasmid that encodes a fusion protein INP-Streptavidin-YFP and one INP-YFP-Streptavidin. Then we would have been able to use the exact same protocol we used to characterize the Biobrick BBa_K523013, to proof surface localization.
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•
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==Sensing/Effector==
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•
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•
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==Device==
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• Cloned one of our Biobricks in a backbone that contains another resistance than chloramphenicol to cotransfect bacteria with the Plasmids responsible for Sensing/Effector and the Plasmid used to connect the Nanoparticles.
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Revision as of 08:44, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Up until now we succeded • Producing Nanoparticles and loading them • Clone a pH sensitive promoter • Engineer a fusion protein between ice nucleation protein and Streptavidin But we weren’t able to characterize and assemble the parts completely.

Contents

What we would have done with more time:

Nanoparticles

• • •

Cell surface display of Streptavidin

• Cloned a plasmid that encodes a fusion protein INP-Streptavidin-YFP and one INP-YFP-Streptavidin. Then we would have been able to use the exact same protocol we used to characterize the Biobrick BBa_K523013, to proof surface localization. • •

Sensing/Effector

• • •

Device

• Cloned one of our Biobricks in a backbone that contains another resistance than chloramphenicol to cotransfect bacteria with the Plasmids responsible for Sensing/Effector and the Plasmid used to connect the Nanoparticles. • •


Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Next_steps"