Team:DTU-Denmark/Notebook/11 July 2013

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(→Combine Nir operon with pZA21)
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===Combine Nir operon with pZA21===
===Combine Nir operon with pZA21===
 +
 +
Mastermix for 7 PCR tubes, Using the mastermix protocol we got from Mathilde.
 +
 +
triplets of each of the two Nir Operon extracts that we have made yesterday, including one negative control:
 +
*Nir 2a(I,II,III)
 +
*Nir 2b(I,II,III)
 +
 +
Using the following PCR program:
 +
{|
 +
! !!time!!Temp C
 +
|-
 +
|Denaturing||2 min||98
 +
|-
 +
| start of loop ||10 sec ||98
 +
|-
 +
|annealing||30 sec||60
 +
|-
 +
|elongation||9 min||72
 +
|-
 +
|goto start of loop 35 times|| ||
 +
|-
 +
|hold|| ||10
 +
|-
 +
|}

Revision as of 12:48, 11 July 2013

Contents

208

Main purpose

Who was in the lab

Ariadni,Henrike,Julia,Jakob

Procedure

Combine Nir operon with pZA21

Mastermix for 7 PCR tubes, Using the mastermix protocol we got from Mathilde.

triplets of each of the two Nir Operon extracts that we have made yesterday, including one negative control:

  • Nir 2a(I,II,III)
  • Nir 2b(I,II,III)

Using the following PCR program:

timeTemp C
Denaturing2 min98
start of loop 10 sec 98
annealing30 sec60
elongation9 min72
goto start of loop 35 times
hold 10
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