Team:Groningen/protocols/Motility assay

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<p>Let the colonies grow over 16 hours.
<p>Let the colonies grow over 16 hours.
<p>After the 16 hour growth period the measure the spread of the colony every 2 hours.
<p>After the 16 hour growth period the measure the spread of the colony every 2 hours.
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<b>Motility Agar 0.7% (100 ml) </b>
<b>Motility Agar 0.7% (100 ml) </b>

Revision as of 11:00, 4 October 2013

Motility assay

Ad 13 ml of the motility agar (0.4% or 0.7%) per plate.

Inoculate the plates at the center with the Strain of interest (strain A).

For every plate inoculated with Strain A inoculate a plate with Wild type (WT).

inoculations is done with 10 ul of liquid culture with an OD600 of 0.4.

Let the colonies grow over 16 hours.

After the 16 hour growth period the measure the spread of the colony every 2 hours.

Motility Agar 0.7% (100 ml)

compound amount treatment
LB broth 2 g
agar 0.7 g

Motility Agar 0.4% (100 ml)

compound amount treatment
LB broth 2 g
agar 0.4 g