"
Team:Tuebingen/Notebook/Protocols/onestep
From 2013.igem.org
(Difference between revisions)
| Line 16: | Line 16: | ||
<div id="actualContent"> | <div id="actualContent"> | ||
| - | |||
| - | |||
<h3>Procedure</h3> | <h3>Procedure</h3> | ||
<p>Dissolve 0.2 M lithium acetate and polyethylene glycol 3350 (PEG 3350). Add 100 mM DTT and filter sterilize. Store in 1 mL aliquots at -20 °C.</p> | <p>Dissolve 0.2 M lithium acetate and polyethylene glycol 3350 (PEG 3350). Add 100 mM DTT and filter sterilize. Store in 1 mL aliquots at -20 °C.</p> | ||
| + | <p> </p> | ||
| + | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | ||
</div> | </div> | ||
Revision as of 12:07, 4 October 2013
ONE-STEP Buffer
Procedure
Dissolve 0.2 M lithium acetate and polyethylene glycol 3350 (PEG 3350). Add 100 mM DTT and filter sterilize. Store in 1 mL aliquots at -20 °C.
Back to Protocols
