Team:MSOE Milwaukee/Primers

From 2013.igem.org

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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase</TD>
   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50 align = left><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG</TD>
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Revision as of 21:01, 11 July 2013

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DNA SEQUENCE/ENZYME PRIMER TO ADD THE PREFIX (FOR) 5' to 3'
PRIMER TO ADD THE SUFFIX (REV) 5' to 3'
1,8-cineole synthase ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC
GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG
HMG-CoA Synthase reverse ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC
GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG
Mevalonate Kinase ATCGGAATTCGCGGCCGCTTCTAGCATGACCAAAAAAGTTGGTGTTGG
GCTAATGATCATCGCCGGCGACGTCCTTATTACAGAGATTCGATCCAGG
Phosphomevalonate Kinase ATCGGAATTCGCGGCCGCTTCTAGCATGATCGCTGTTAAAACCTGC
GCTAATGATCATCGCCGGCGACGTCCTTATTAAGATTTGTCGTCGTGACC
Mevalonate Diphosphate Kinase ATCGGAATTCGCGGCCGCTTCTAGCATGGACCGTGAACCGG
GCTAATGATCATCGCCGGCGACGTCCTTATTAGCAGCAGTCGTCC
Xylanase C ATCGGAATTCGCGGCCGCTTCTAGCATGTTCTGCTCTAACGCTCAGC
GCTAATGATCATCGCCGGCGACGTCCTTATTAGGTCAGACCCATTTTTTCC
Xylosidase ATCGGAATTCGCGGCCGCTTCTAGCATGAAATCTAAACTGCTGTTCCCG
GCTAATGATCATCGCCGGCGACGTCTTATTACGGTTTTTTGGTCAGG
osmY with GS liner (two reverse) ATCGGAATTCGCGGCCCGCTTCTAGATGACTATGACAAGACTGAAG
GGATCCCCCGCTACCACTGCCCGAACCCTTAGTTTTCAGATCATT
GCTAATGATCATCGCCGGCGACGTCGGATCCCCCGCTACCACTGCCC

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