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Team:Groningen/Labwork/12 July 2013
From 2013.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
<br> Made 3 bottles of 250 ml LB Agar (20g/L LB, 16g/L Agar) | <br> Made 3 bottles of 250 ml LB Agar (20g/L LB, 16g/L Agar) | ||
<br> Autoclaved: | <br> Autoclaved: | ||
| - | <br> 3 bottles of demiwater(80 ml) | + | <br> 3 bottles of demiwater (80 ml) |
<br> 3 bottles of tabwater (80 ml) | <br> 3 bottles of tabwater (80 ml) | ||
| - | <br> 3 bottles of | + | <br> 3 bottles of milliQ (80 ml) |
| - | <br> 3 bottles of LB Agar | + | <br> 3 bottles of LB Agar (250 ml) |
| - | <br> 1 bottle of | + | <br> 1 bottle of milliQ (1 L) |
Revision as of 10:12, 12 July 2013
Mirjam Made a PCR with (1) no strep tag (2) strep F (3) strep R. The annealing temperature for (1) and (2) is 78 degrees Celsius. For (3) the annealing temperature is 80 degrees Celsius.
Gel purification of the strep F silk obtained yesterday.
Run a 0.8% agarose gel for 30 min at 90V.
Inne
Made 3 bottles of 250 ml LB Agar (20g/L LB, 16g/L Agar)
Autoclaved:
3 bottles of demiwater (80 ml)
3 bottles of tabwater (80 ml)
3 bottles of milliQ (80 ml)
3 bottles of LB Agar (250 ml)
1 bottle of milliQ (1 L)
