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Team:Heidelberg/Templates/Delftibactin week3
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| + | ===Precipitation of Gold from Electronic Waste=== | ||
| + | ====Protocol==== | ||
| + | In order to evaluate optimial cultivation temperature of ''D. acidovorans'', glycerol stock was streaked on ACM plates and incubated at RT, 30°C and 37°C ON. The next day, plates were covered with 200 µl 0.5% soft agar supplemented with 27 µl "dissolved electronic waste" solution. | ||
| + | ====Result==== | ||
Revision as of 18:18, 4 October 2013
Contents |
13-05 - 19-05-13
Gold Recovery from Electronic Waste
As test experiment, pins from an old CPU processor chip were dissolved.
 Fig.0 Old motherboard from which we will recycle gold. |
Step 1: Pins were mechanically removed and collected in a flask.
 Fig.1.1 CPU from which pins will be removed. |
 Fig.1.2 Removed pins. |
Step 2: Flask was put onto heating plate and covered in concentrated HCl.
 Fig.2 Pins covered in HCl. |
Step 3: HNO3 was carefully added to the pins and heated.
 Fig.3 Evolution NO2 gas. |
Step 4: After several minutes of moderate heating, pins dissolved completely. The solution turned green.
 Fig.4 Green solution of dissolved pins. |
Step 5: The solution was carefully neutralized to pH ~6 in a step-wise manner using first 5 M, later 0.5 M NaOH.
Step 6: This led to precipitation of copper salts, indicated by the milky green coloring.
 Fig.6 Milky solution following neutralization. |
Step 7: In order to remove the precipitates, the solution was filtered using a buechner funnel and sterile filtrated (0.2 µm).
 Fig.7.1 Filtering solution to remove precipitates. |
 Fig.7.2 Sterile filtering. |
 Fig.7.3 Filters used for sterile filteration. |
Step 8: The final yellow-green gold containing solution was collected in a volumetric flask.
 Fig.8Final gold containing solution. |
Precipitation of Gold from Electronic Waste
Protocol
In order to evaluate optimial cultivation temperature of D. acidovorans, glycerol stock was streaked on ACM plates and incubated at RT, 30°C and 37°C ON. The next day, plates were covered with 200 µl 0.5% soft agar supplemented with 27 µl "dissolved electronic waste" solution.
