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Team:Heidelberg/Templates/Del week17 overview
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(Created page with " ==Validation of pFSN== We screened for more colonies with pFSN but we didn't find any more positive ones. <br/><br/> Furthermore we did some tests for the validation of pFSN i...") |
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==Validation of pFSN== | ==Validation of pFSN== | ||
| - | We screened for more colonies with pFSN but we didn't find any more positive | + | We screened for more colonies with pFSN but we didn't find any more positive clones. <br/><br/> |
Furthermore we did some tests for the validation of pFSN in colony D8w. | Furthermore we did some tests for the validation of pFSN in colony D8w. | ||
Revision as of 18:25, 4 October 2013
Validation of pFSN
We screened for more colonies with pFSN but we didn't find any more positive clones.
Furthermore we did some tests for the validation of pFSN in colony D8w.
| Validation of pFSN in clone D8w | ||
|---|---|---|
| Method | Details method | Result |
| Screening for inserted fragments | Fragments pSB4K5 and DelA-F |  |
| Fragments DelA-F and DelF-G | | |
| Restriction Digest | EcoRI | |
| BamHI | | |
| BglII | | |
| Sequencing gibson overlaps | Primer FS_13_short | |
| Primer FS_22 | | |
| Primer VFII | | |
| Primer VR |  | |
There is a mutation at the beginning of the mRFP cassette. This explains why the colonies do not get red. All other tests for valiation were positive. Consequently colony D8w includes the right plasmid. Because we cannot use mRFP as expression control we will do SDS-PAGE instead to prove that the proteins are expressed.
