Team:NTNU-Trondheim/Primers
From 2013.igem.org
(Difference between revisions)
Line 101: | Line 101: | ||
<td><b> R_CFP_tat</tr></b></td> | <td><b> R_CFP_tat</tr></b></td> | ||
<td>5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3'</td> | <td>5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3'</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th> SYFP</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b> F_I_syfp</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b> R_syfp</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th> BFP</th> | ||
+ | <tr> | ||
+ | <td><b> F_tat_BFP</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>R_I_bfp</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th> GFP</th> | ||
+ | <tr> | ||
+ | <td><b>F_tat_GFP</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>R_I_gfp</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th> RFP</th> | ||
+ | <tr> | ||
+ | <td><b>F_I_rfp</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>R_pl.b_rfp</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th> GFPmut3</th> | ||
+ | <tr> | ||
+ | <td><b>F_tat_GFPmut3</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>R_pl.b_GFPmut3</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th> CFP</th> | ||
+ | <tr> | ||
+ | <td><b>F_tat_CFP</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>R_l_CFP</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th> YFP</th> | ||
+ | <tr> | ||
+ | <td><b>F_l_YFP</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>R_pl.b_YFP</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th> Protein G</th> | ||
+ | <tr> | ||
+ | <td><b>F_pl.b_PrG</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>F_tat_PrG</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>F_pl.b_pelB_PrB</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>R_pl.b_PrG</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>R_pl.b_PrGstop</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th> Plasmid backbone</th> | ||
+ | <tr> | ||
+ | <td><b>F_plasmid </tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>F_RFP_pl.b</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>F_YFP_pl.b</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>R_pl.b</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>R_tat_pl.b</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th> Sequancing Primers</th> | ||
+ | <tr> | ||
+ | <td><b>F_sequence</tr></b></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><b>R_sequence</tr></b></td> | ||
+ | <td></td> | ||
</tr> | </tr> | ||
Revision as of 18:39, 4 October 2013
![header](https://static.igem.org/mediawiki/2013/5/55/Logo-ntnu.png)
Primers
tat-signal sequence | |
---|---|
Primer | Sequance |
F_pl.b_tat | 5'- AGAGAAAGAGGAGAAATACTAGatggccaataacgatctctttcaggcatcacg-3' |
F_prefix_tat | 5'- GAATTCGCGGCCGCTTCTAGatggccaataacgatctctttcaggcatcacg-3' |
R_tat | 5'-cgcttgcgccgcagtcgcacgtcg-3' |
R_GFP_tat | 5'-CTTCTCCTTTACGCATcgcttgcgccgcagtcgcacgtcg -3' |
R_CFP_tat | 5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3' |
SYFP | |
F_I_syfp | |
R_syfp | |
BFP | |
F_tat_BFP | |
R_I_bfp | |
GFP | |
F_tat_GFP | |
R_I_gfp | |
RFP | |
F_I_rfp | |
R_pl.b_rfp | |
GFPmut3 | |
F_tat_GFPmut3 | |
R_pl.b_GFPmut3 | |
CFP | |
F_tat_CFP | |
R_l_CFP | |
YFP | |
F_l_YFP | |
R_pl.b_YFP | |
Protein G | |
F_pl.b_PrG | |
F_tat_PrG | |
F_pl.b_pelB_PrB | |
R_pl.b_PrG | |
R_pl.b_PrGstop | |
Plasmid backbone | |
F_plasmid | |
F_RFP_pl.b | |
F_YFP_pl.b | |
R_pl.b | |
R_tat_pl.b | |
Sequancing Primers | |
F_sequence | |
R_sequence |