Team:NTNU-Trondheim/Primers
From 2013.igem.org
(Difference between revisions)
Line 130: | Line 130: | ||
<tr> | <tr> | ||
<td><b>F_tat_GFP</tr></b></td> | <td><b>F_tat_GFP</tr></b></td> | ||
- | <td></td> | + | <td>5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3' </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_I_gfp</tr></b></td> | <td><b>R_I_gfp</tr></b></td> | ||
- | <td></td> | + | <td>5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3'</td> |
</tr> | </tr> | ||
Line 141: | Line 141: | ||
<tr> | <tr> | ||
<td><b>F_I_rfp</tr></b></td> | <td><b>F_I_rfp</tr></b></td> | ||
- | <td></td> | + | <td>5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_pl.b_rfp</tr></b></td> | <td><b>R_pl.b_rfp</tr></b></td> | ||
- | <td></td> | + | <td>5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3'</td> |
</tr> | </tr> | ||
Line 152: | Line 152: | ||
<tr> | <tr> | ||
<td><b>F_tat_GFPmut3</tr></b></td> | <td><b>F_tat_GFPmut3</tr></b></td> | ||
- | <td></td> | + | <td>5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_pl.b_GFPmut3</tr></b></td> | <td><b>R_pl.b_GFPmut3</tr></b></td> | ||
- | <td></td> | + | <td>5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3'</td> |
</tr> | </tr> | ||
Line 163: | Line 163: | ||
<tr> | <tr> | ||
<td><b>F_tat_CFP</tr></b></td> | <td><b>F_tat_CFP</tr></b></td> | ||
- | <td></td> | + | <td>5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_l_CFP</tr></b></td> | <td><b>R_l_CFP</tr></b></td> | ||
- | <td></td> | + | <td>5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3'</td> |
</tr> | </tr> | ||
Revision as of 19:19, 4 October 2013
![header](https://static.igem.org/mediawiki/2013/5/55/Logo-ntnu.png)
Primers
tat-signal sequence | |
---|---|
Primer | Sequence |
F_pl.b_tat | 5'- AGAGAAAGAGGAGAAATACTAGatggccaataacgatctctttcaggcatcacg-3' |
F_prefix_tat | 5'- GAATTCGCGGCCGCTTCTAGatggccaataacgatctctttcaggcatcacg-3' |
R_tat | 5'-cgcttgcgccgcagtcgcacgtcg-3' |
R_GFP_tat | 5'-CTTCTCCTTTACGCATcgcttgcgccgcagtcgcacgtcg -3' |
R_CFP_tat | 5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3' |
SYFP | |
F_I_syfp | 5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggttagcaagggcgaaga-3' |
R_syfp | 5'-GCCTTTCGTTTTATTTGATGCCTGGttattatttatacagctcatccatacccaggg-3' |
BFP | |
F_tat_BFP | 5'- CGACGTGCGACTGCGGCGCAAGCGatgagcgaactgatcaaagag-3' |
R_I_bfp | 5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCattcagtttatgacccagcttgc-3' |
GFP | |
F_tat_GFP | 5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3' |
R_I_gfp | 5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3' |
RFP | |
F_I_rfp | 5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3' |
R_pl.b_rfp | 5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3' |
GFPmut3 | |
F_tat_GFPmut3 | 5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3' |
R_pl.b_GFPmut3 | 5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3' |
CFP | |
F_tat_CFP | 5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3' |
R_l_CFP | 5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3' |
YFP | |
F_l_YFP | |
R_pl.b_YFP | |
Protein G | |
F_pl.b_PrG | |
F_tat_PrG | |
F_pl.b_pelB_PrB | |
R_pl.b_PrG | |
R_pl.b_PrGstop | |
Plasmid backbone | |
F_plasmid | |
F_RFP_pl.b | |
F_YFP_pl.b | |
R_pl.b | |
R_tat_pl.b | |
Sequencing Primers | |
F_sequence | |
R_sequence |