Team:Groningen/Lab/experiments/Biofilm

From 2013.igem.org

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The suggested implant material was placed in a petri dish with SGG medium. The material is partially submerged in the medium and inoculated with the appropriate <B.subtilis</i> strain. After incubation the plate is left in a 27&deg;C environment for 5 days. After the incubation time, the total biofilm growth on the material of interest is measured. A first test shows that after 5 days there is 3 mm biofilm, started from the medium.  
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The suggested implant material was placed in a petri dish with SGG medium. The material is partially submerged in the medium and inoculated with the appropriate <B.subtilis</i> strain. After incubation the plate is left in a 27&deg;C environment for 5 days. After the incubation time, the total biofilm growth on the material of interest is measured.
<br>Two different implant materials are tested: titanium and plastic. After five days of growth on titanium, there is 3 mm biofilm, starting at the medium growing up on titanium. No biofilm formation was possible on plastic.  
<br>Two different implant materials are tested: titanium and plastic. After five days of growth on titanium, there is 3 mm biofilm, starting at the medium growing up on titanium. No biofilm formation was possible on plastic.  

Revision as of 22:59, 4 October 2013

Biofilm Growth on different materials

To find out if the Bacillus subtilis strain used in this project can form a biofilm on material often used as implants, an experiment was set up.

The suggested implant material was placed in a petri dish with SGG medium. The material is partially submerged in the medium and inoculated with the appropriate strain. After incubation the plate is left in a 27°C environment for 5 days. After the incubation time, the total biofilm growth on the material of interest is measured.
Two different implant materials are tested: titanium and plastic. After five days of growth on titanium, there is 3 mm biofilm, starting at the medium growing up on titanium. No biofilm formation was possible on plastic.


Figure 1: Titanium plate with 3 mm growth.


Figure 2: Plastic in Sgg medium, no growth on the plasic came about.