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Exeter/4 July 2013
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(Created page with " == Morning == * Tried to make clear agar using : 5g NaCl } 15g Tryptone } Makes 1 litre 15g Agar } 5g Soytone } Tryptone, NaCl and so...")
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(Created page with " == Morning == * Tried to make clear agar using : 5g NaCl } 15g Tryptone } Makes 1 litre 15g Agar } 5g Soytone } Tryptone, NaCl and so...")
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Revision as of 10:35, 15 July 2013
Morning
- Tried to make clear agar using :
5g NaCl } 15g Tryptone } Makes 1 litre 15g Agar } 5g Soytone }
Tryptone, NaCl and soytone were added into a mixer with a magnetic stirrer. (was a yellow colour).
3g of agar were added to two 200ml durans. The Tryptone mix was then added to the durans, filling it up to 200ml.
Mixture was yellow, not colourless - the experiment was not successful. Durans put in the autoclave in the evening to let the agar set overnight.
Afternoon
- Transformation of competent cells
