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Team:Heidelberg/Templates/Indigoidine week22
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(Created page with " ===BioBrick Characterization=== ====Test Digest==== We digested 400 ng of each of our parts [http://parts.igem.org/Part:BBa_K1152008 BBa_K1152008] to BBa_K1152019 with EcoRI...")
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(Created page with " ===BioBrick Characterization=== ====Test Digest==== We digested 400 ng of each of our parts [http://parts.igem.org/Part:BBa_K1152008 BBa_K1152008] to BBa_K1152019 with EcoRI...")
Newer edit →
Revision as of 00:37, 5 October 2013
BioBrick Characterization
Test Digest
We digested 400 ng of each of our parts [http://parts.igem.org/Part:BBa_K1152008 BBa_K1152008] to BBa_K1152019 with EcoRI, XbaI, SpeI and PstI. We used 0.5 ul enzyme and incubated the reaction at 37 °C for 2 hours. We submitted the plasmids pKH6-10, pRB22, pRB23, pRB23-T8, -T10, -T12, -T13 and -T14.
20130925 indBB dig.png
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The digest is positive and the fragment sizes are correct. Together with the sequencing results we can be sure that our submitted parts are alright.
