Team:Heidelberg/Templates/Indigoidine week22
From 2013.igem.org
(Difference between revisions)
(Created page with " ===BioBrick Characterization=== ====Test Digest==== We digested 400 ng of each of our parts [http://parts.igem.org/Part:BBa_K1152008 BBa_K1152008] to BBa_K1152019 with EcoRI...") |
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We submitted the plasmids pKH6-10, pRB22, pRB23, pRB23-T8, -T10, -T12, -T13 and -T14. | We submitted the plasmids pKH6-10, pRB22, pRB23, pRB23-T8, -T10, -T12, -T13 and -T14. | ||
<gallery widths="400px"> | <gallery widths="400px"> | ||
- | + | File:Heidelberg_20130925_indBB_dig.png | |
</gallery> | </gallery> | ||
The digest is positive and the fragment sizes are correct. Together with the sequencing results we can be sure that our submitted parts are alright. | The digest is positive and the fragment sizes are correct. Together with the sequencing results we can be sure that our submitted parts are alright. |
Latest revision as of 00:55, 5 October 2013
BioBrick Characterization
Test Digest
We digested 400 ng of each of our parts [http://parts.igem.org/Part:BBa_K1152008 BBa_K1152008] to BBa_K1152019 with EcoRI, XbaI, SpeI and PstI. We used 0.5 ul enzyme and incubated the reaction at 37 °C for 2 hours. We submitted the plasmids pKH6-10, pRB22, pRB23, pRB23-T8, -T10, -T12, -T13 and -T14.
The digest is positive and the fragment sizes are correct. Together with the sequencing results we can be sure that our submitted parts are alright.