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Team:Heidelberg/Templates/DelH week25
From 2013.igem.org
(Difference between revisions)
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==HM17-FS_78== | ==HM17-FS_78== | ||
Annealing temps: 62°C, 60°C, 57°C, 55°C <br/> | Annealing temps: 62°C, 60°C, 57°C, 55°C <br/> | ||
| - | Elongation time: 2:10 | + | Elongation time: 2:10 <br/> |
| + | --> Did not work | ||
==Screening remaining Gibson electroporated== | ==Screening remaining Gibson electroporated== | ||
60 colonies were picked and screened with primers FS_73 and FS_95 (annealing 66 touchdown, 62) | 60 colonies were picked and screened with primers FS_73 and FS_95 (annealing 66 touchdown, 62) | ||
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| Gibson Master Mix 2x || 10 | | Gibson Master Mix 2x || 10 | ||
|} | |} | ||
| + | |||
| + | ==ccdB== | ||
| + | Annealing temps: </br> | ||
| + | 12 cycles 74, 68, 64 and 58°C </br> | ||
| + | 18 cycles 72, 66, 62 and 56°C | ||
Revision as of 15:26, 18 October 2013
(18.10)
Contents |
HM17-FS_78
Annealing temps: 62°C, 60°C, 57°C, 55°C
Elongation time: 2:10
--> Did not work
Screening remaining Gibson electroporated
60 colonies were picked and screened with primers FS_73 and FS_95 (annealing 66 touchdown, 62)
Gibson assembly
| Reagent | Volume added (µl) |
|---|---|
| BB (FS..) | 0.61 |
| DN11-FS66 | 1.00 |
| FS_67-FS_68 | 1.35 |
| FS_69-FS_70 | 6.34 |
| FS_71-HM08 | 0.70 |
| Gibson Master Mix 2x | 10 |
ccdB
Annealing temps: </br> 12 cycles 74, 68, 64 and 58°C </br> 18 cycles 72, 66, 62 and 56°C
