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Template:Team:SydneyUni Australia/Calendar/Events List
From 2013.igem.org
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title: 'Meeting Yagiz', | title: 'Meeting Yagiz', | ||
start: new Date(2013, 4, 3), | start: new Date(2013, 4, 3), | ||
| - | description: '<b>Members:</b> Nick, Rob, Andrew, Viv, Shuravi, Cyril, Desmond, Hugh, Evelyn<br> <b>What we did: </b>Met with Yagiz and Rob from the MQ | + | description: '<b>Members:</b> Nick, Rob, Andrew, Viv, Shuravi, Cyril, Desmond, Hugh, Evelyn<br> <b>What we did: </b>Met with Yagiz and Rob from the MQ University iGEM team for a general introduction to iGEM and some helpful advice. ' |
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// -----------------Early days 7 ---------------- | // -----------------Early days 7 ---------------- | ||
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title: 'Chloride Assay', | title: 'Chloride Assay', | ||
start: new Date(2013, 4, 31), | start: new Date(2013, 4, 31), | ||
| - | description: '<b>Members:</b> | + | description: '<b>Members:</b> Coleman, Rob, Hugh<br> <b>What we did: </b> After 24 hours in DCA, we put E. Coli (pBBR) in the cool room for a Cl assay next week. We washed and transferred E. Coli (pBS-ToMO) from broth to two phosphate buffer treatments, one with DCA, one without. We also ran a quick PCR testing our new ToMO primers on pBS-ToMO, but we didn’t digest pBS-ToMO before PCR. !' |
}, | }, | ||
// -----------------Week 3 ---------------- | // -----------------Week 3 ---------------- | ||
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title: 'Meeting with Yagiz', | title: 'Meeting with Yagiz', | ||
start: new Date(2013, 5, 2), | start: new Date(2013, 5, 2), | ||
| - | description: '<b>Members:</b> Rob <br> <b>What we did: </b> | + | description: '<b>Members:</b> Rob <br> <b>What we did: </b> We met with Yagiz at Macquarie University to talk about Strange Nature and possible collaboration with our iGEM teams.' |
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// -----------------Week 6 ---------------- | // -----------------Week 6 ---------------- | ||
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title: 'PCR of pBBR-mcs2 Origin', | title: 'PCR of pBBR-mcs2 Origin', | ||
start: new Date(2013, 6, 11), | start: new Date(2013, 6, 11), | ||
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title: 'PCR and Diagnostic Gel', | title: 'PCR and Diagnostic Gel', | ||
start: new Date(2013, 8, 5), | start: new Date(2013, 8, 5), | ||
| - | description: '<b>Members:</b> Andrew <br> <b>What we did: </b>We did a PCR of dhlB from the GA reaction products using three samples (p-p, p-a and a negative control containing no DNA)<br><br>All GA enzymes were heat-killed by placing using thermocycler at | + | description: '<b>Members:</b> Andrew <br> <b>What we did: </b>We did a PCR of dhlB from the GA reaction products using three samples (p-p, p-a and a negative control containing no DNA)<br><br>All GA enzymes were heat-killed by placing using thermocycler at 95 degrees.<br><br>We ran a gel of dhlB PCR of Gibson assembly products. The resulting bands were clear and as expected.' |
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title: 'Plasmid Prep, PCR Screen', | title: 'Plasmid Prep, PCR Screen', | ||
start: new Date(2013, 8, 10), | start: new Date(2013, 8, 10), | ||
| - | description: '<b>Members:</b> Andrew <br> <b>What we did: </b>A plasmid prep was performed on pSB-RFP, taken from transformed E.coli TOP10 cells. Stopped at precipitation stage.<br><br>We received our new set of primers! We used these to PCR screen all our selected Gibson assembly plasmids and GA restriction products and our positive GA control (puc19- | + | description: '<b>Members:</b> Andrew <br> <b>What we did: </b>A plasmid prep was performed on pSB-RFP, taken from transformed E.coli TOP10 cells. Stopped at precipitation stage.<br><br>We received our new set of primers! We used these to PCR screen all our selected Gibson assembly plasmids and GA restriction products and our positive GA control (puc19-dhlA-dhlB)<br><br>To confirm that our PCR screening had worked we ran a gel of all our PCR products' |
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title: 'Ligation and Transformation', | title: 'Ligation and Transformation', | ||
start: new Date(2013, 8, 12), | start: new Date(2013, 8, 12), | ||
| - | description: '<b>Members:</b> Rob, Andrew, Vivian <br> <b>What we did: </b>Today a digestion of pSB-rfp was made using the enzymes, xbaI and PstI for ligation. Our PCR fragments (dhlA and dhlB) were also digested using the same restriction enzymes.<br><br>Following purification of our PCR DNA we ligated our inserts (dhlA, dhlB or | + | description: '<b>Members:</b> Rob, Andrew, Vivian <br> <b>What we did: </b>Today a digestion of pSB-rfp was made using the enzymes, xbaI and PstI for ligation. Our PCR fragments (dhlA and dhlB) were also digested using the same restriction enzymes.<br><br>Following purification of our PCR DNA we ligated our inserts (dhlA, dhlB or dhlA-dhlB) into our plasmid vector (pSB). We split the reaction volume to two and use one later by storing in the cool room.<br><br>The ligated products were transformed into E.coli Top10 and EpI300 and plated on to LB-Cm.' |
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title: 'Plasmid Prep, More Screening', | title: 'Plasmid Prep, More Screening', | ||
start: new Date(2013, 8, 14), | start: new Date(2013, 8, 14), | ||
| - | description: '<b>Members:</b> Rob, Andrew <br> <b>What we did: </b>Based on yesterday screens we selected clones for plasmid prepping. Plasmids were digested using | + | description: '<b>Members:</b> Rob, Andrew <br> <b>What we did: </b>Based on yesterday screens we selected clones for plasmid prepping. Plasmids were digested using EcoRV to check length and concentration of DNA.<br><br>To find successfully ligated dhlB we did more PCR screening. This time however we used EPI300 cells instead of TOP10. Screening was also done for dhlB-dhlA.' |
}, | }, | ||
// -----------------Week 13 ---------------- | // -----------------Week 13 ---------------- | ||
Revision as of 16:21, 22 October 2013
