Team:XMU-China/Content parts

From 2013.igem.org

(Difference between revisions)
Line 480: Line 480:
<tr><td>Fig.7-6 AGE confirmation of plasmid pSB4K5-ndh construction
<tr><td>Fig.7-6 AGE confirmation of plasmid pSB4K5-ndh construction
</td></tr>
</td></tr>
-
 
-
 
-
 
-
 
-
<div class="clear"></div>
 
-
 
-
<div id="home_s4"><center>
 
-
<img src="https://static.igem.org/mediawiki/2013/c/cb/Xmusoftware_sponsor1.png" width="80px" height="80px">
 
-
<img src="https://static.igem.org/mediawiki/2013/1/18/Xmusoftware_sponsor2.png" width="80px" height="80px">
 
-
<img src="https://static.igem.org/mediawiki/2013/6/6f/Xmu-Bg_header_mwlogo_notag.jpg" height="60px">
 
-
<img src="https://static.igem.org/mediawiki/2013/e/ed/Xmu-evernote.jpg" height="60px">
 
-
</center>
 
-
</div>
 
-
<div id="footer-menu">
 
-
<div class="center-block clearfix">
 
-
<ul id="second-menu">
 
-
<li><a href="https://2013.igem.org/Team:XMU-China">Home</a></li>
 
-
<li><a href="https://2013.igem.org/Team:XMU-China/Team1">Team</a></li>
 
-
<li><a href="https://2013.igem.org/Team:XMU-China/Background">Project</a></li>
 
-
<li><a href="https://2013.igem.org/Team:XMU-China/Notebook">Notebook</a></li>
 
-
<li><a href="https://2013.igem.org/Team:XMU-China/Software">Software</a></li>
 
-
<li><a href="https://2013.igem.org/Team:XMU-China/Human Practice">Human Practice</a></li>
 
-
<li><a href="https://2013.igem.org/Team:XMU-China/Safety">Safety</a></li>
 
-
<li style="margin-left: 400px"><a href="https://2013.igem.org/Team:XMU-China/Team1#Attribution">Contact us</a></li>
 
-
</ul>
 
-
<div class="copyright" style="margin-right: -150px">&copy; Copyright 2013.XMU-China iGEM All rights reserved.</div>
 
-
</div><!-- .center-block -->
 
-
</div><!-- #footer-menu -->
 
-
<!-- END FOOTER MENU -->
 
-
</body>
 
-
</html>
 

Revision as of 17:09, 26 October 2013

LinkUp - Multipurpose HTML Template

Parts

Table 1. The Bio-bricks used in the study

Part

Backbone

Type

Location

Size (bp)

Description

part

backbone

BBa_K546000

pSB1C3

Signaling

2013-P1-12D

1964

2070

Lux pL controlled LuxR with lux pR autoinducing LuxI (lva tag)- AHL.

BBa_I763020

pSB1C3

Intermediate

RBS-GFP-TT

2013-P3-11H

914

2070

RBS-GFP (+LVA Tag)-Terminators

BBa_F2621

pSB1A2

Signaling

2013-P2-21F

1158

2079

3OC6HSL Receiver Device

BBa_K546001

pSB1C3

Device

2013-P1-12F

2135

2070

AIIA

AHL Reporter and Quencher

BBa_J04450

pSB4K5

Reporter

2013-P5-5G

1069

3419

RFP Coding Device

BBa_J04450

pSB3T5

Reporter

2013-P5-7C

1069

3241

RFP Coding Device

BBa_J23022

BBa_J23006

Composite

2013-P5-3H

234

2356

[key3d][TT]


Favorite XMU-China 2013 iGEM Team Parts

W/N Name Type Description Designer Length
W BBa_K1036000 Composite lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II Shengquan Zeng
Zhaopeng Cheng		 2687
W BBa_K1036001 Coding the ndh gene coding for respiratory NADH dehydrogenase II Shengquan Zeng
Zhaopeng Cheng		 1366
W BBa_K1036003 Composite lux pL controlled luxR with lux pR controlled gfp (LVA-tag) Xin Guo
Xi Xi		 4052
W BBa_K1036006 Composite lux pL controlled luxR with lux pR controlled sfgfp (with LVA-tag) Zhaopeng Cheng
Xiyu Wu		 4046


XMU-China 2013 iGEM Team Parts Sandbox

W/N Name Type Description Designer Length
W BBa_K1036002 Composite gfp (with LVA-tag) under plux R control Xin Guo
Xi Xi		 2866
W BBa_K1036004 Reporter sfgfp with LVA-tag Zhaopeng Cheng
Xiyu Wu		 753
W BBa_K1036005 Composite sfgfp (with LVA-tag) under plux R control Zhaopeng Cheng
Xiyu Wu		 2880


Agarose Gel Electrophoresis Confirmation for all Plasmids

pSB1C3-gfp-luxI

This plasmid consists of two parts of target genes: gfp and luxI, and both of them are regulated by quorum sensing promoter. High copy number. (Fig.7-1)

Fig.7-1 AGE confirmation of plasmid pSB1C3-gfp-luxI construction

pSB1C3-sfgfp-luxI

This plasmid consists of two parts of target genes: sfgfp and luxI, and both of them are regulated by quorum sensing promoter. sfGFP gene is included into this plasmid as a parallel experiment group of pSB1C3-gfp-luxI. High copy number. (Fig.7-2)

Fig.7-2 AGE confirmation of plasmid pSB1C3-sfgfp-luxI construction

p3H-GFP-luxI

This plasmid consists of two parts of target genes: gfp and luxI, and both of them are regulated by quorum sensing promoter. Middle copy number. (Fig.7-3)

Fig.7-3 AGE of p3H-gfp construction

p3H-sfGFP-luxI

This plasmid consists of two parts of target genes: sfgfp and luxI, and both of them are regulated by quorum sensing promoter. sfGFP gene is included into this plasmid as a parallel experiment group of pSB1C3-gfp-luxI. Middle copy number. (Fig.7-4)

Fig.7-4 AGE of p3H-sfgfp construction

pSB3T5-aiiA

This plasmid has one target gene aiiA under the regulation of quorum sensing promoter. Middle copy number. (Fig. 7-5)

Fig.7-5 AGE confirmation of plasmid pSB3T5-aiiA construction

pSB4K5-ndh

This plasmid has one target gene aiiA under the regulation of quorum sensing promoter. Low copy number. (Fig. 7-6)

Retrieved from "http://2013.igem.org/Team:XMU-China/Content_parts"
Fig.7-6 AGE confirmation of plasmid pSB4K5-ndh construction