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Team:British Columbia/Notebook
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==Team Caffeine== | ==Team Caffeine== | ||
Revision as of 04:40, 17 July 2013
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Contents |
Team CRISPR
July 3
List of BioBricks that are/will be on the way:
Cas9:
- Cas9 - Joe and Anna
- pTet -
- const. promoter + Cas9 - Joe
- promoter + Cas9 + Leader + R + spacer + R (on Kan)
- L + R + spacer + R
Target:
- tracerRNA
- repeat + spacer + repeat - Vanins*2 + underlings
- leader - Ray, Fisal, Dan
Decoy:
- PAM + spacer (on Amp)
Team cinnamaldehyde
- 4-coumarate-CoA ligase:
PCR worked
Team Caffeine
June 20
Joe, Dan, and I made heat-shock competent cells today - they can be found in the top compartment of the -80º Freezer #3 in the room full of freezers (thanks Cam!). We will be ordering primers (through Diane) to modify the TU Munich caffeine biosynthesis BioBricks we got in the Distribution Kit.
We're making a start - what are the other groups up to?
--grace.yi
