iGEM Team Heidelberg 2013

Ilia Kats

One of our team members, the secret nerd in chief of our group, Ilia Kats, had the initial idea for our project and inspired us all with his dream of easy and fast recycling of gold. He also came up with the concept to exploit the NRPS modularity for setting up the NRPS designer.
Besides all of his brain work, Ilia was indispensable for all of the wetlab work for the different sub projects. He was single-handedly responsible for cloning the methylmalonyl-CoA patwhay for the Delftibactin project. In the Synthetic Peptides, Indigoidine-Tag and Tag-Optimization subproject he was involved in the pilot experiments. And last but not least, his programming skills were essential for the successful implementation of our NRPSDesigner software where he used his supernatural force to eliminate even the toughest bugs.
A big THANK U from the whole team for the inspiration and great humor.

Synthetic Peptides

The main focus of this project was to demonstrate the feasibility of NRPS module shuffling. This was performed by Joshua Sachs, Philipp Walch, Tania Christiansen and Anja Riedel. They established the experimental strategy and designed all corresponding primers needed for the amplification of the different Tyrocidine modules. Tania Christiansen was especially responsible for the BioBrick submission and the cloning of the fragments into the needed RFCs.
Another essential part in the Synthetic Peptides project was the mass spectrometry of which Joshua Sachs was in charge. Everyone in this group contributed to the great success of this part of the project! Here, we want to thank our advisor Lorenz Adlung, who helped us with his great experience he shared in frequent lab-meeting discussions.

Indigoidine-Tag

This subproject was about developing an easily detectable, inert and universal tag for NRPs. The work on the fusion peptides comprising modules from the Tyrocidine cluster were performed by Joshua Sachs, Philipp Walch, Tania Christiansen and Anja Riedel. Philipp Walch and Joshua Sachs performed the valine-indigoidine as well as the di- and tripeptide-indigoidine tagging, Anja Riedel took care of the linker variation. The ccdB-construct was generated by Joshua Sachs and Philipp Walch. Without the devotion of all of the people mentioned above, the project would not have been feasible.
We want to thank Dominik Niopek and Hanna Meyer for their expertise they shared with us when we were working on the RFC100.

Tag Optimization

Ralf Beer, Konrad Herbst and Nikolaos Ignatiadis designed strategies for the optimization of the Indigoidine-Tag, which includes the creation of 58 engineered indigoidine synthetases and the development of a quantitative indigoidine assay. These hard-working fellows did not mind spending 48 hours in a row at the bench, cloning large plasmid libraries and performing hundreds of co-transformations, thereby establishing the RFC99. Konrad showed that one can write a script for almost any purpose. He contributed most to the evaluation of the indigoidine assay, the improvement of the Tag Optimization strategy and the RFC99. Nikos was involved in the design of the first constructs and was a great help in the creation of the synthetic T-domains. Ralf focussed on plasmid and primer design as well as the labwork and was actually considered to be integrated into the NRPSDesigner as an alternative sequence based domain prediction tool. But not only these three guys were infected by the blue fever - thanks to Hannah Meyer they always had an advisor at their side with whom they had many creative scientific and even more non-scientific but still creative discussions.

Gold Recycling

The cloning strategies and amplification of the Del cluster were performed and developed by Florian Schmidt, Sophie Rabe, Nils Kurzawa and Johanna Meichsner. The experiments started with the amplification of DelH executed by Johanna Meichsner which turned out to be one of the most challenging parts of our project. Johanna proved to be extremely resilient to recurrent setbacks and her power of endurance gave a great example to the whole team. Florian Schmidt, Sophie Rabe and Nils Kurzawa took care of the plasmid assembly of the remaining genes from the Del cluster. After DelRest was succesfully amplified, Florian Schmidt and Sophie Rabe worked as well on the assembly of the DelH plasmid and the gold precipitation by Delftibactin. Sophie Rabe was furthermore responsible of proteins assays, delftibactin purification and MICRO-TOF, Florian Schmidt entablished the protocol to dissolve electronic waste. All of them were extremely dedicated to their work and cloned day and night.
Ilia Kats cloned the pIK8 plasmid and all prior plasmids necessary for the establishment of the methylmalonyl-CoA pathway. He performed all steps of the cloning procedure of these plasmids from primer design up to electroporation and screening.
Here we want to give a special thank you to our advisors Dominik Niopek, Fanny Georgi and Katharina Genreith for their great help in planning the cloning strategies and introducing us to the world of primer design! Thanks to Fanny for helping us with electroporations any time of the day (or night).

NRPSDesigner

The software-core-team consisted of Konrad Herbst, Ilia Kats, Nikolaos Ignatiadis, Julia Schessner and Nils Kurzawa. They worked together on the implementation of the NRPSDesigner software and the establishment of the respective database. Additionally, Joshua Sachs, Anja Riedel and Philipp Walch contributed to the maintenance of the database. Furthermore, Ilia Kats was the administrator of our internal server. All of the people mentioned above worked on our sub-software project iGEM42. The advisors Hannah Meyer and Tim Heinemann helped, not only with the NRPSDesigner, but also with motivating the whole team. A big thanks to Hannah for baking all those cookies and to Tim for helping to eat them!

Wiki Design

Our wiki is uses stylesheets from Bootstrap 3.0 and was implemented by Nils Kurzawa. He wrote the main javascripts and css style sheets used on this wiki. Nils created the main page together with the project pages and all templates. Tania Christiansen was responsible for all the pictures used for the wiki navigation. All team members worked together to generate the interface graphics and basic figures in inkskape. Especially Tania Christiansen and Anja Riedel could bring in their talent. Thanks also to our creative advisors Fanny Georgi and Katharina Genreit who came up with many ideas. After the basic implementation, Nils received help in the wiki design from Nikolaos Ignatiadis, Ilia Kats, Konrad Herbst and Johanna Meichsner. Important to mention is again Tim Heinemann here for his niggling but always helpful comments.

Human Practice

The entire team worked on our human practice projects. Philipp Walch is a member of the education initiative "Life-Science Lab" and organized the essay competition for the high school students. Philipp Walch, Sophie Rabe and Tania Christiansen presented our project at various events to the interested public together with members of the high school iGEm Team from 2012. Joshua Sachs who volunteers as a fire fighter established the contact with the army which gave us insight in the bioweapons defense program (ABC). Ralf Beer and Konrad Herbst received valuable input from Peptide Specialties Laboratories GmbH. Philipp Walch and Nikos Ignatiadis visited TBM Edelmetall Recycling. Johanna Meichsner introduced us to Dorothea van Aaken, who organizes environmental workshops for primary students. Fanny Georgi organized the contact to Dr. Zawatzky from German Cancer Research Center (DKFZ). Dominik Niopek introduced us to the science author Olaf Fritsche and Lorenz Adlung, who also writes, got us in contact with Isabel Marleen Pötzsch. Both Dominik Niopek and Lorenz Adlung also helped with the "not invented by nature" collaboration.

BioQuant, University of Heidelberg

Prof. Dr. Thomas Höfer

Prof. Dr. Thomas Höfer's group provided us a sample of E. coli BL21 (DE3) (pLys).

Thi Bach Nga Ly-Hartig

Nga Ly-Hartig got us in touch with several research groups working with mass spectrometry and supported us with great cupcakes.

Prof. Dr. Robert B. Russel

Prof. Russel supplied us with a vial of E. coli BL21 (DE3).

Dr. Oliver Wichmann

Dr. Wichmann supported us with his equipment especially by allowing us to use his lyophilizer.

Center for Molecular Biology Heidelberg (ZMBH), University of Heidelberg

Core Facility for Mass Spectronomy and Proteomics

Dr. Thomas Ruppert and Dr. Bernd Hessling helped us with Mass Spectrometry measurements for the detection of our non-ribosomal peptides.

Dr. Oliver Gruss

The Gruss Lab kindly provided us the E. coli BL21 (DE3) (pLys) strain.

Department of Biochemistry, Philipps-University Marburg

Prof. Dr. Mohamed Marahiel

Prof. Dr. Marahiel kindly provided us with the Brevibacillus parabrevis ATCC 8185 strain and contributed helpful feedback on NRPS and our NRPSDesigner.

Department of Biosystems Science and Engineering, ETH Zurich

Prof. Dr. Martin Fussenegger

Prof. Fussenegger send us the expression plasmids for bpsA and svp, codon optimized for mammalian cells.

Department of Chemical and Biological Engineering, University of Buffalo

Prof. Blaine Pfeifer

Lei Fang from the Pfeifer Lab kindly provided us with the E. coli BAP1 strain and the pLF03 and pKD46 plasmids.

ESRC Centre for Economic and Social Aspects of Genomics (Cesagen), Lancaster University, Institute of Advanced Studies (UNU-IAS), United Nations University

Dr. Paul Oldham

For kindly supporting us with his expertise in information processing, interpretation of the social aspects of synthetic biology and for sharing his collection of synthetic biology terms with us.

German Cancer Research Center (DKFZ), Heidelberg

Prof. Dr. Stephan Herzig

Prof. Herzig and his group kindly offered the possibility to use their electroporator.

Dr. Dirk Nettelbeck

Dr. Nettelbeck, Ph.D.contributed with valuable input on homologous recombination.

Prof. Dr. Rainer Zawatzky

Prof. Zawatzky gave us lots of feedback on safety considerations and ethical concerns.

Harvard Wyss Institute for Biologically Inspired Engineering, Center for Life Sciences, Boston

Dr. Matthew Mattozzi

Dr. Mattozzi supported us with valuable advice on genomic homologous recombination. Dr. Mattozzi also kindly provided the pET21c-pcc-acc-mmce plasmid. His PhD student Marika Ziesack, M.sc. kindly introduced us to Gibson Cloning.

Heidelberg Technology Park UniTT Start-Up Centre, University of Heidelberg

Dr. Raoul Haschke

Dr. Haschke opened up new economical perspectives on the possible industrial scale NRP production and theidea to start our own company.

Institute of Chemical Engineering, University of Vienna

Dipl.-Ing.Paul Kroll

Mr. Kroll contributed with great scientific input on modelling the industrial scale Delftibactin production in E. coli.

Institute of Organic Chemistry, University of Heidelberg

Mass Spectrometry Facility

Prof. Groß kindly provided us with the possibility to use his HR-ESI MS and helped with data analysis.

Institute of Pharmacology and Molecular Biotechnology (IPMB), University of Heidelberg

Prof. Dr. Christian Klein and Lena Weigel

Prof. Klein kindly allowed us to use the MICRO-TOF of his group. Special thanks to Lena Weigel who helped us with the operation.

Dr. Holger Schäfer

Dr. Schäfer supported us with scientific and moral support during the cloning of the Del constructs.

Dr. Richard Wombacher

Marcel Best from Dr. Wombacher's group saved us with the last minute supply of a rotary evaporator!

Peptide Specialties Laboratories GmbH, Heidelberg

Dr. Hans-Richard Rackwitz

Dr. Rackwitz gave us fascinating insights in chemical peptide synthesis and valuable scientific input on our project.

School of Medicine, University of Maryland

Prof. Jacques Ravel, Ph.D.

Professor Jacques Ravel continuously helped us with the automated domain prediction pipeline of the NRPSDesigner software, for example by providing us with seed alignments for the Hidden Markov Models of his own PKS/NRPS analysis tool . He also tested the NRPSDesigner software and gave us valuable feedback.

University Medical Center Heidelberg, University of Heidelberg

Neonate Screening Facility

Dr. Dirk Kohlmüller and Dr. Kathrin Schmidt supported us in amino acid level detection by mass spectrometry.