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Team:Groningen/Labwork/17 July 2013
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<br/>Miniprep for the samples of the 4th plate (they grew O/N). Restriction analysis with EcoRV. | <br/>Miniprep for the samples of the 4th plate (they grew O/N). Restriction analysis with EcoRV. | ||
<br/>Run gel of the restriction analysis. | <br/>Run gel of the restriction analysis. | ||
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| + | '''Inne''' | ||
| + | <br>The e.coli cells with the BBa_k818000 that were to grow overnight didn't grow. | ||
| + | <br>The tubes that were supposed to be turbid were still clear, however the negative control was also clear so that one did succeed. | ||
| + | |||
| + | <br>Autoclaved new tips (white yellow and blue) and PCR tubes. | ||
| + | |||
| + | <br>Made new 0.8% Agarose solution. | ||
Revision as of 10:11, 17 July 2013
Mirjam
Restriction digest of the 1st and 3rd plate colonies are examined on gel. 5/7 colonies are correct transformations. To be completely sure if the transformation did work, a PCR for the promoter is made, using the colonies and the original construct as a backbone.
Miniprep for the samples of the 4th plate (they grew O/N). Restriction analysis with EcoRV.
Run gel of the restriction analysis.
Inne
The e.coli cells with the BBa_k818000 that were to grow overnight didn't grow.
The tubes that were supposed to be turbid were still clear, however the negative control was also clear so that one did succeed.
Autoclaved new tips (white yellow and blue) and PCR tubes.
Made new 0.8% Agarose solution.
