Team:Groningen/Labwork/17 July 2013

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'''Mirjam'''
'''Mirjam'''
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<br/>Restriction digest of the 1st and 3rd plate colonies are examined on gel. 5/7 colonies are correct transformations. To be completely sure if the transformation did work, a PCR for the promoter is made, using the colonies and the original construct as a backbone.  
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<br/>Check of the restriction done yesterday. This revealed that 5/7 colonies did contain the transformant. The cells of the fourth plate started to grow, so a mini prep analysis is performed. A restriction analysis is done with EcoRV.
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<br/>Miniprep for the samples of the 4th plate (they grew O/N). Restriction analysis with EcoRV.
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A second confirmation of the constructs is done using PCR.
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<br/>Run gel of the restriction analysis.
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'''Inne'''
'''Inne'''
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<br>The e.coli cells with the BBa_k818000 that were to grow overnight didn't grow.
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<br>The ''E. coli'' cells with the BBa_k818000 that were to grow overnight didn't grow.
<br>The tubes that were supposed to be turbid were still clear, however the negative control was also clear so that one did succeed.
<br>The tubes that were supposed to be turbid were still clear, however the negative control was also clear so that one did succeed.

Revision as of 10:13, 17 July 2013

Mirjam
Check of the restriction done yesterday. This revealed that 5/7 colonies did contain the transformant. The cells of the fourth plate started to grow, so a mini prep analysis is performed. A restriction analysis is done with EcoRV.

A second confirmation of the constructs is done using PCR.

Inne
The E. coli cells with the BBa_k818000 that were to grow overnight didn't grow.
The tubes that were supposed to be turbid were still clear, however the negative control was also clear so that one did succeed.


Autoclaved new tips (white yellow and blue) and PCR tubes.


Made 400 mL 0.8% Agarose solution.

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