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Team:DTU-Denmark/Notebook/19 June 2013
From 2013.igem.org
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==Transformations== | ==Transformations== | ||
| - | *[http://parts.igem.org/Part:BBa_I0500 I0500] | + | *from plate 3: [http://parts.igem.org/Part:BBa_I0500 I0500] |
| - | * | + | *from plate 3: [http://parts.igem.org/Part:BBa_K808000 K808000] |
| - | + | *from plate 3: [http://parts.igem.org/Part:BBa_K206001 K206001] | |
| + | *from plate 3: [http://parts.igem.org/Part:BBa_K564012 K564012] | ||
| + | *from plate 3: [http://parts.igem.org/Part:BBa_I13458 I13458] | ||
| + | *from plate 5: [http://parts.igem.org/Part:BBa_I13458 I13458] | ||
Revision as of 12:42, 17 July 2013
208
PCR of pZE21
Mastermix:
- dNTP 5ul
- HF Buffer 50ul
- polymerase 2.5ul
- water 157.5ul
- fw primer (1A) 15ul
- rv primer 1B 15ul
- temlate 1ul/tube
Transformations
- from plate 3: [http://parts.igem.org/Part:BBa_I0500 I0500]
- from plate 3: [http://parts.igem.org/Part:BBa_K808000 K808000]
- from plate 3: [http://parts.igem.org/Part:BBa_K206001 K206001]
- from plate 3: [http://parts.igem.org/Part:BBa_K564012 K564012]
- from plate 3: [http://parts.igem.org/Part:BBa_I13458 I13458]
- from plate 5: [http://parts.igem.org/Part:BBa_I13458 I13458]
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