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Team:HokkaidoU Japan/Shuffling Kit/Primer Designer
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Revision as of 15:26, 28 October 2013
Maestro E.coli
Optimization Kit
Primer Designer for Maestro
1. First, you have to decide whether or not you conform to our default overhang set.
2. Secondly, you hove to determine how many CDS are included in your target plasmid.
I have CDS(s) included.3. Then, please select the region to where your fragment correspond in target plasmid.
1
2
3
8
9
4. Okey, now we are ready to go. Enter your fragment's sequence, and press "Calculate"!
2. Please enter overhangs and fragment's sequence, and press "Calculate"
Forward
- sequence
- tm
Reverse
- sequence
- tm
