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Team:Groningen/Labwork/18 July 2013
From 2013.igem.org
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<br/>prepared 20 tubes for use in -80 c Glyserol stock | <br/>prepared 20 tubes for use in -80 c Glyserol stock | ||
| - | '''Inne''' | + | '''Inne & Mirjam''' |
<br>Cells with BBa_k818000, inoculated overnight, were successfully grown. | <br>Cells with BBa_k818000, inoculated overnight, were successfully grown. | ||
<br>Both negative controls were clear, and the rest of the samples was turbid. | <br>Both negative controls were clear, and the rest of the samples was turbid. | ||
Revision as of 09:23, 18 July 2013
Sander
prepared 20 tubes for use in -80 c Glyserol stock
Inne & Mirjam
Cells with BBa_k818000, inoculated overnight, were successfully grown.
Both negative controls were clear, and the rest of the samples was turbid.
Did a miniprep of the samples 1 & 2 (the ones with Amp).
Afterwards concentration of DNA was determined via nanodrop.
Results: sample 1 60.1 ng/uL, sample 2 31.5 ng/uL
During the miniprep the filtered LB medium was poured back into the culture (so cells could grow again).
A -80 C stock was made with 1 mL culture (sample 1).
