Team:Groningen/Labwork/18 July 2013

From 2013.igem.org

(Difference between revisions)
Line 2: Line 2:
<br/>prepared 20 tubes for use in -80 c Glyserol stock.
<br/>prepared 20 tubes for use in -80 c Glyserol stock.
<br/>created a restriction digest reaction for silk.
<br/>created a restriction digest reaction for silk.
 +
<table border="1">
 +
<tr>
 +
<td>Sample</td>
 +
<td>componant</td>
 +
<td>volume (ul) <td>
 +
</tr>
 +
<tr>
 +
<td>silk1</td>
 +
<td>silk</td>
 +
<td>4</td>
 +
</tr>
 +
<tr>
 +
<td></td>
 +
<td>BamHI buffer</td>
 +
<td>2</td>
 +
</tr>
 +
<tr>
 +
<td></td>
 +
<td>BamHI enzyme</td>
 +
<td>0,5</td>
 +
</tr>
 +
<tr>
 +
<td></td>
 +
<td>BSA</td>
 +
<td>0,5</td>
 +
</tr>
 +
</table>
 +
<br/>MilliQ was added to reach a 20 ul volume.
 +
<br/> placesin  incubater at 37,2 C at 17:10

Revision as of 15:18, 18 July 2013

Sander
prepared 20 tubes for use in -80 c Glyserol stock.
created a restriction digest reaction for silk.

Sample componant volume (ul)
silk1 silk 4
BamHI buffer 2
BamHI enzyme 0,5
BSA 0,5


MilliQ was added to reach a 20 ul volume.
placesin incubater at 37,2 C at 17:10


Inne & Mirjam
Cells with BBa_k818000, inoculated overnight, were successfully grown.
Both negative controls were clear, and the rest of the samples was turbid.
Did a miniprep of the samples 1 & 2 (the ones with Amp).

# composition Bacteria
1 4mL LB 8uL Amp +
2 4mL LB 8uL Amp +
3 4mL LB 8uL Amp (negative control) -
4 4mL LB +
5 4mL LB (negative control) -


Afterwards concentration of DNA was determined via nanodrop.


Results: sample 1 = 60.1 ng/uL, sample 2 = 31.8 ng/uL


During the miniprep the filtered LB medium was poured back into the culture (so cells could grow again).
A -80 C stock was made with 1 mL culture (sample 1). (stored in tray: iGEM 2012 A, compartment 70)

Retrieved from "http://2013.igem.org/Team:Groningen/Labwork/18_July_2013"