Revision as of 15:21, 18 July 2013

208

Gosia, Henrike

- miniprep of RFP in pZA21 and cycAX in pZA21

- made gycerol stocks from ON cultures supposedly containing RFP in pZA21 and cycAX in pZA21

- gel of yesterday's PCR (to get AMO with USER endings) -> got the fragment

- purified AMO for USER from gel

- PCR to add His-tags to construct 1 (Sec-GFP-RFP) and construct 2 (TAT-GFP-RFP)

- gel on today's PCR -> construct 1 has no product, construct 2 has an unkown product with length 900 bp

1% agarose gel of today's PCR

Revision as of 15:21, 18 July 2013 (view source) Hezscha (Talk \| contribs) (→Procedure) ← Older edit		Revision as of 15:21, 18 July 2013 (view source) Hezscha (Talk \| contribs) (→Results) Newer edit →
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	1% agarose gel of today's PCR		1% agarose gel of today's PCR

-	[[File:2013-07-17 his construct.jpg\| ~~200px~~]]	+	[[File:2013-07-17 his construct.jpg\| 600px]]