From 2013.igem.org
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| - | {{:Team:ZJU-China/template/reset}}
| + | #REDIRECT [[Team:ZJU-China/Project/TheGhostKit/GhostSensor#Detect_thrombin_using_.CE.B2-lactamase_testing_strip]] |
| - | {{:Team:ZJU-China/template/page_css}}
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| - | {{:Team:ZJU-China/template/header}}
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| - | <html>
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| - | <div id="main-image"></div>
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| - | <div class="container">
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| - | <div id="container-wrapper">
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| - | </html>{{:Team:ZJU-China/template/project-menu|GhostSensor=Checked}}<html>
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| - | <div class="content">
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| - | == Bacterial Ghost: Perspectives ==
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| - | Our ghost sensor platform can not only be used to detect proteins, it can also be used to specifically clear proteins in solution and achieve long-term storage of proteins.
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| - | === Magic strip ===
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| - | For the second set of inner-membrane scaffolds, two parts of β-lactamase were fused to the periplasmic ends. When 19-mer and 25-mer biotin-modified aptamers are added to the solution and thrombin is present, aptamers act like a bridge linking streptavidin-fused membrane scaffolds and thrombin. In this way, two molecules of membrane scaffolds are pulled together and the two residuals of β-lactamase will meet each other again to become an integrity part with enzymatic activity. This activity can be detected by commercial β-lactamase testing strips, with a total preparation time of about 10 min.
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| - | <center>
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| - | [[File:P30928-104814.jpg|600px]]
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| - | β-lactamase testing strip. Left shows β-lactamase positive, right shows negative.
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| - | </center>
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| - | <html>
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| - | <div style="float:left;">Previous:
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| - | <a href="./Results">Bacterial Ghost: Results</a>
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| - | </div>
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| - | <div style="float:right;">Next:
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| - | <a href="../TheGhostKit/GhostSensor">The Ghost Kit: Ghost Sensor</a>
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| - | </div>
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| - | </div>
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| - | </div>
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| - | <div class="clean"></div>
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| - | </div>
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| - | </html>{{:Team:ZJU-China/template/footer}}
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Latest revision as of 03:01, 29 October 2013
- REDIRECT Team:ZJU-China/Project/TheGhostKit/GhostSensor#Detect_thrombin_using_.CE.B2-lactamase_testing_strip
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