Team:UCSF/Project/Circuit/Design1
From 2013.igem.org
(Difference between revisions)
Line 529: | Line 529: | ||
<div id="photos" style = "width: 300px; float:right" align="justify"> | <div id="photos" style = "width: 300px; float:right" align="justify"> | ||
<center><img style="margin-top:0px; height:250px"; padding:0;" | <center><img style="margin-top:0px; height:250px"; padding:0;" | ||
- | + | src="https://static.igem.org/mediawiki/2013/2/25/UCSF_HighPromoterModel.jpg"> </center> | |
- | + | ||
- | src="https://static.igem.org/mediawiki/2013/ | + | |
- | + | ||
- | "> </center> | + | |
</div> | </div> | ||
+ | |||
Revision as of 03:38, 29 October 2013
CRISPR Decision-Making Circuit
Promoter Sensitivity:
For the summer, we used fluorescent proteins to differentiate between our target cell strains and our unaffected cell strains. Our targeted cells will be marked with red fluorescent protein (RFP) while our unaffected cells with be marked with the fluorescent protein, citrine. Both cell strains will receive the conjugative plasmid from the donor. The gRNA-dCAS9 complex will then form and repress the production of RFP in our target cells. The RFP cell strain will no longer be able to fluoresce, since the gRNA in our conjugative plasmid only recognizes a specific site on RFP, while the citrine cell strain will be left unaffected because there is no gRNA in the conjugative plasmid that recognizes citrine.