Team:Hong Kong CUHK/results
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<p>After 10 to 12 hours of incubation at 37 °C, laccase showed maximum activity when 0.3 mM IPTG was used for introducing protein expression (Figure 2).</p> | <p>After 10 to 12 hours of incubation at 37 °C, laccase showed maximum activity when 0.3 mM IPTG was used for introducing protein expression (Figure 2).</p> | ||
<p align="center"><a href="http://parts.igem.org/File:La1.jpg"><img alt="La1.jpg" src="https://static.igem.org/mediawiki/2013/a/a5/800px-La1.jpg" width="600" ></a></p> | <p align="center"><a href="http://parts.igem.org/File:La1.jpg"><img alt="La1.jpg" src="https://static.igem.org/mediawiki/2013/a/a5/800px-La1.jpg" width="600" ></a></p> | ||
- | <p align="center">Figure 2. Laccase | + | <p align="center">Figure 2. Laccase activity monitoring</p> |
<p> </p> | <p> </p> | ||
<h3>3. Laccase function verification</h3> | <h3>3. Laccase function verification</h3> | ||
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<h3>1. Biobrick DNA length verification</h3> | <h3>1. Biobrick DNA length verification</h3> | ||
<p align="center"><img src="https://static.igem.org/mediawiki/parts/archive/8/8d/20130928021743%21Di.jpg" height="400"></p> | <p align="center"><img src="https://static.igem.org/mediawiki/parts/archive/8/8d/20130928021743%21Di.jpg" height="400"></p> | ||
- | <p align="center">Figure 4. Restriction | + | <p align="center">Figure 4. Restriction digestion plasmid gel picture</p> |
<h3>2. Catechol 1,2-dioxygenase function verification by time series degradation assay</h3> | <h3>2. Catechol 1,2-dioxygenase function verification by time series degradation assay</h3> | ||
<p>Catechol 1,2-dioxygenase is resposible for phenol degredation (Naiem <em>et al</em>., 2011). Before it is integrated into our PAHs degrdation system, hydroquinone, also known as benzene-1,4-diol or quinol, which is an aromatic organic compound belonging to the phenol family, was used as a substrate to test Catechol 1,2-dioxygenase expression and activity.</p> | <p>Catechol 1,2-dioxygenase is resposible for phenol degredation (Naiem <em>et al</em>., 2011). Before it is integrated into our PAHs degrdation system, hydroquinone, also known as benzene-1,4-diol or quinol, which is an aromatic organic compound belonging to the phenol family, was used as a substrate to test Catechol 1,2-dioxygenase expression and activity.</p> |
Revision as of 03:44, 29 October 2013