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Team:Groningen/Labwork/23 July 2013
From 2013.igem.org
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'''Mirjam''' | '''Mirjam''' | ||
<br/>Run a gel with the PCR products of silk without strep + signal sequence. | <br/>Run a gel with the PCR products of silk without strep + signal sequence. | ||
| - | <br/>The PCR of silk without strep + signal sequence is successful. A new 1.5% agarose gel is made to do gel purification. | + | <br/>The PCR of silk without strep + signal sequence is successful. A new 1.5% agarose gel is made to do gel purification. Did the gel purification. |
Run a gel with the purified PCR product of silk strepF-R and silk F-strepR. | Run a gel with the purified PCR product of silk strepF-R and silk F-strepR. | ||
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Made a restriction digestion for the signal sequences. | Made a restriction digestion for the signal sequences. | ||
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| + | '''Sander''' | ||
| + | Made a restriction digestion for silk strepF-R and silk F-strepR. | ||
Revision as of 09:03, 23 July 2013
Mirjam
Run a gel with the PCR products of silk without strep + signal sequence.
The PCR of silk without strep + signal sequence is successful. A new 1.5% agarose gel is made to do gel purification. Did the gel purification.
Run a gel with the purified PCR product of silk strepF-R and silk F-strepR.
The products are present in 2/3 samples for each silk type. These are combined and concentrated. Concentrations of 18.1 and 16.1 ng/ul are obtained. So a restriction digestion can be made.
Made a restriction digestion for the signal sequences.
Sander Made a restriction digestion for silk strepF-R and silk F-strepR.
