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Team:DTU-Denmark/Notebook/7 June 2013
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->in 1 of these L, 14 g of agar were added for making LB solid medium | ->in 1 of these L, 14 g of agar were added for making LB solid medium | ||
| - | ==plating E.coli== | + | ===plating E.coli=== |
*Colonies of Top 10 E.coli were streaked on two plates | *Colonies of Top 10 E.coli were streaked on two plates | ||
*Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan) | *Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan) | ||
Revision as of 12:48, 23 July 2013
07 June 2013
Contents |
lab 208
Main purposes today
- helloworld-project
- prepare solutions
- autoclaving
- plate E.coli
Who was in the lab
Kristian
Procedure
- 1M CaCl_2 (200ml) weighed off 22.206 g CaCl_2
- 50% glycerol (500 ml)
- LB medium (liquid) (3 L);
->20 g of LB in every 1 L of distilled water. Autoclaved medium
->in 1 of these L, 14 g of agar were added for making LB solid medium
plating E.coli
- Colonies of Top 10 E.coli were streaked on two plates
- Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)
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