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Team:DTU-Denmark/Notebook/23 July 2013
From 2013.igem.org
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==colony PCR gel== | ==colony PCR gel== | ||
| + | |||
| + | 1% gel to analyse yesterday's colony PCR | ||
| + | |||
| + | wells: | ||
| + | |||
| + | *1: 1 kb ladder | ||
| + | *2: HAO colony 1 | ||
| + | *3: HAO colony 2 | ||
| + | *4: RFP in pZA21 without promoter | ||
| + | *5: RFP in pZA21 without promoter | ||
| + | *6: AMO colony 3 (count starts from 3) | ||
| + | *7: AMO colony 4 | ||
| + | *8: AMO colony 5 | ||
| + | *9: AMO colony 6 | ||
| + | *10: AMO colony 7 | ||
| + | *11: AMO colony 8 | ||
| + | *12: AMO colony 9 | ||
| + | *13: AMO colony 10 | ||
| + | *break in the gel | ||
| + | *14: cycAX colony 1 | ||
| + | *15: cycAX colony 2 | ||
| + | *14: RFP colony 1 | ||
| + | *14: RFP colony 2 | ||
[[File:2013-07-23 colony cyc rfp.jpg|600px]] | [[File:2013-07-23 colony cyc rfp.jpg|600px]] | ||
Revision as of 11:41, 24 July 2013
Results
Verification gel
1% gel to verify our earlier PCR purifications
wells (in bracets position of the purification in the gene box):
- 1: broad band ladder
- 2: HAO extracted from N. europeae with flanking primers (A4)
- 3: AMO extracted from N. europeae with flanking primers (A6)
- 4: cycAX extracted from N. europeae with flanking primers (A5)
- 5: Nir extracted from P. aeruginosa with flanking primers (A7)
- 6: Nir extracted from P. aeruginosa with flanking primers (A8)
- 7: Nir extracted from P. aeruginosa with flanking primers (B8)
- 8: Nir extracted from P. aeruginosa with flanking primers (B9)
- 9: pZA21 USER fragment, DpnI treated (F1)
- 10: cycAX USER fragment (G1)
- 11: AMO USER fragment (G2)
- 12: HAO USER fragment (G3)
- 13: Nir USER fragment (G4)
- 14: AMO USER fragment (G5)
- 15: 1kb plus ladder
colony PCR gel
1% gel to analyse yesterday's colony PCR
wells:
- 1: 1 kb ladder
- 2: HAO colony 1
- 3: HAO colony 2
- 4: RFP in pZA21 without promoter
- 5: RFP in pZA21 without promoter
- 6: AMO colony 3 (count starts from 3)
- 7: AMO colony 4
- 8: AMO colony 5
- 9: AMO colony 6
- 10: AMO colony 7
- 11: AMO colony 8
- 12: AMO colony 9
- 13: AMO colony 10
- break in the gel
- 14: cycAX colony 1
- 15: cycAX colony 2
- 14: RFP colony 1
- 14: RFP colony 2
