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Revision as of 12:52, 24 July 2013 (view source) Hezscha (Talk | contribs) (→208) ← Older edit		Revision as of 12:55, 24 July 2013 (view source) Hezscha (Talk | contribs) (→208) Newer edit →
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	===USER reaction and transformation ===		===USER reaction and transformation ===
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		+	We perform USER reaction in order to create construct of plasmid pZA21 and AMO and other construct - pZA21 and HAO. We add also negative control with water instead of insert.
		+	Reaction is performed according to our protocol with increased amount of insert.
		+
	===Restriction analysis===		===Restriction analysis===
	===Primer diluting===		===Primer diluting===

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Revision as of 12:55, 24 July 2013

Contents 1 208 1.1 Main purpose 1.2 Who was in the lab 1.3 Procedure 1.3.1 USER reaction and transformation 1.3.2 Restriction analysis 1.3.3 Primer diluting 1.3.4 PCR to extract Nir from Pseudosomonas with new USER primers 1.4 Results 1.5 Conclusion

208

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Main purpose

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Who was in the lab

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Henrike, Julia, Gosia, Krystian

Procedure

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USER reaction and transformation

We perform USER reaction in order to create construct of plasmid pZA21 and AMO and other construct - pZA21 and HAO. We add also negative control with water instead of insert. Reaction is performed according to our protocol with increased amount of insert.

Restriction analysis

Primer diluting

PCR to extract Nir from Pseudosomonas with new USER primers

Results

Conclusion

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