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Team:DTU-Denmark/Notebook/24 July 2013
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We perform restriction analysis with EcoRI. Expected fragments are as follows: | We perform restriction analysis with EcoRI. Expected fragments are as follows: | ||
| - | * | + | |
| + | * For pZA21 with AMO: | ||
| + | - 3309 pz | ||
| + | - 2283 pz | ||
| + | |||
| + | * For pZA21 with HAO: | ||
| + | - 2233 pz | ||
| + | - 2124 pz | ||
| + | - 930 pz | ||
| + | |||
===Primer diluting=== | ===Primer diluting=== | ||
===PCR to extract Nir from Pseudosomonas with new USER primers=== | ===PCR to extract Nir from Pseudosomonas with new USER primers=== | ||
Revision as of 13:52, 24 July 2013
Contents |
208
Main purpose
Who was in the lab
Henrike, Julia, Gosia, Krystian
Procedure
USER reaction and transformation
We perform USER reaction, samples are as follows:
- plasmid pZA21 and AMO
- plasmid pZA21 and HAO
- negative control with water instead of insert
Reaction is performed in the same way as on 18-07-2013 with increased amount of insert (up to 14 uL due to low DNA concentration).
Restriction analysis
From last week transformants with AMO and HAO in pZA21 we performed plasmid isolation.
We perform restriction analysis with EcoRI. Expected fragments are as follows:
- For pZA21 with AMO:
- 3309 pz - 2283 pz
- For pZA21 with HAO:
- 2233 pz - 2124 pz - 930 pz
Primer diluting
PCR to extract Nir from Pseudosomonas with new USER primers
Results
Conclusion
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