"
Team:DTU-Denmark/Notebook/25 July 2013
From 2013.igem.org
(Difference between revisions)
(→Gel 1) |
(→208) |
||
| Line 17: | Line 17: | ||
===gel electrophoresis=== | ===gel electrophoresis=== | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
===ON cultures and re-plating of Nir USER transformants from Colony PCR=== | ===ON cultures and re-plating of Nir USER transformants from Colony PCR=== | ||
| Line 53: | Line 38: | ||
# 1 kb ladder | # 1 kb ladder | ||
| + | [[File:2013-07-25_AMO_HAO_and_restriction_cyc_EcoRI_HindIII.jpg|600px]] | ||
| - | + | ===Gel 2=== | |
| - | + | ||
| - | + | ||
==Conclusion== | ==Conclusion== | ||
Revision as of 12:51, 25 July 2013
Contents |
208
Main purpose
- PCR of AMO and HAO using USER primers and as a template using AMO and HAO extracted from chromosomal DNA with non-uracil primers
- verification of PCRs
- ON cultures and re-plating of Nir USER transformants from Colony PCR ( 17-07-2013)
Who was in the lab
Kristian, Gosia, Julia
Procedure
PCR in order to amplify AMO and HAO with USER primers
gel electrophoresis
ON cultures and re-plating of Nir USER transformants from Colony PCR
2 positive Nir USER transformants obtained by colony PCR were inoculated in 5 mL LB medium + 30 ug/ml kanamicyn for ON cultures preparation
Results
Gel 1
- 1 kb ladder
- AMO 5uL template, 1
- AMO 5uL template, 2
- AMO 10uL template, 1
- AMO 10uL template, 2
- HAO 5uL template, 1
- HAO 5uL template, 2
- HAO 10uL template, 1
- HAO 10uL template, 2
- restriction analysis cyc EcoRI
- restriction analysis cyc HindIII
- 1 kb ladder
Gel 2
Conclusion
Navigate to the Previous or the Next Entry
