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Team:DTU-Denmark/Notebook/14 June 2013
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*PCR was run | *PCR was run | ||
*transformation of [http://parts.igem.org/Part:BBa_I0500 Biobrick I0500] on Amp instead of Kan | *transformation of [http://parts.igem.org/Part:BBa_I0500 Biobrick I0500] on Amp instead of Kan | ||
Revision as of 13:27, 25 July 2013
14 June 2013
208
- PCR was run
- transformation of [http://parts.igem.org/Part:BBa_I0500 Biobrick I0500] on Amp instead of Kan
- transformation of [http://parts.igem.org/Part:BBa_K564012 Biobrick K564012] on CAM
- we used two different batches of competent cells and 200 ul of cells instead of 50ul
- we incubated on LB rather than SOC
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