Team:Groningen/protocols/GelElectrophoresis

From 2013.igem.org

(Difference between revisions)
Line 26: Line 26:
<h3>Materials:</h3>
<h3>Materials:</h3>
<ul type="square">
<ul type="square">
-
<li></li>
+
<li>Power supply</li>
-
<li></li>
+
<li>Agarose gel</li>
-
<li></li>
+
<li>Tray</li>
-
<li></li>
+
<li>DNA samples</li>
-
<li></li>
+
<li>DNA Ladder</li>
</ul>
</ul>
-
<h3>:</h3>
+
<h3>Procedure:</h3>
-
 
+
<br>All the DNA samples are mixed 1:1 ratio with Loading Dye 6x.
-
<br>All the reagents are added following the order listed in the table above.
+
<br>The samples are loaded on agarose gel 0.8% or 1.5% (diluted in TBE buffer 1x).
-
<br>After the reaction is ready mix the content of the tube and spin it down.
+
<br>90V electric potential is applied to the gel as long as the bands are apart from each other (30-45 minutes).
-
<br>The tubes are incubated for 1h at 37&deg;C.
+
<br>The gel are run in TBE buffer 1x.
-
<br>
+
</div>
</div>

Revision as of 10:45, 26 July 2013

Gel electrophoresis

Materials:

  • Power supply
  • Agarose gel
  • Tray
  • DNA samples
  • DNA Ladder

Procedure:


All the DNA samples are mixed 1:1 ratio with Loading Dye 6x.
The samples are loaded on agarose gel 0.8% or 1.5% (diluted in TBE buffer 1x).
90V electric potential is applied to the gel as long as the bands are apart from each other (30-45 minutes).
The gel are run in TBE buffer 1x.