Team:Groningen/protocols/GelElectrophoresis

From 2013.igem.org

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Line 35: Line 35:
<h3>Procedure:</h3>
<h3>Procedure:</h3>
All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
-
<br>The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb gene ruler of Fermentas.
+
<br>The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb Gene Ruler of Fermentas.
<br>A 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.
<br>A 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.
<br>The gel is run in TBE buffer 1x.
<br>The gel is run in TBE buffer 1x.

Revision as of 17:26, 27 July 2013

Gel electrophoresis

Materials:

  • Power supply
  • Agarose gel
  • Tray
  • DNA samples
  • DNA Ladder

Procedure:

All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb Gene Ruler of Fermentas.
A 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.
The gel is run in TBE buffer 1x.